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In order to produce the IgG portion of the Anti-Human Globulin, rabbits are immunised with purified human IgG. Any non-specific activity in the rabbit serum is removed by absorption. The anti-C3d component is an IgM antibody prepared from culture supernatants of a hybridoma derived from the spleen cells of an immunised mouse and a mouse myeloma cell. The rabbit anti-IgG and monoclonal
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